Growth of Chlamydia trachomatis in McCoy Cells Treated with Cytochalasin B
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چکیده
منابع مشابه
Growth of Chlamydia trachomatis in McCoy cells treated with cytochalasin B.
When the fungal metabolite cytochalasin B was added to McCoy cells, multinucleated giant cells developed. Monolayers of these cells proved as efficient as irradiated cells for the growth of three different serotypes of Chlamydia trachomatis and for the primary isolation of chlamydiae from clinical specimens obtained from patients attending a venereal disease clinic. Cytochalasin treatment of Mc...
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An isolation technique for Chlamydia trachomatis using McCoy cells is described. In contrast to earlier techniques employing such cells, no pretreatment of the cells was used. The glutarimide antibiotic cycloheximide was added to the culture medium used for incubating the cells after infection. Cycloheximide was used at concentrations that depressed, but did not completely inhibit, the metaboli...
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The sensitivity of non-replicating McCoy cells pretreated with polyethylene glycol for the isolation of Chlamydia trachomatis from clinical specimens and for the growth of a laboratory strain was compared with the sensitivity of untreated non-replicating cell cultures. The concentration of polyethylene glycol in different solutions and the time of addition to the cell culture medium were critic...
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The 24-hour delay between seeding coverslips with cells and inoculating samples for culture of chlamydiae was reduced to less than 1 hour by using coverslips which had been pre-treated with glutaraldehyde-activated gamma-aminopropyl-triethoxysilane. Treated coverslips were not toxic for McCoy cells and even one year after treatment monolayers formed rapidly on them. Furthermore, all of 13 Chlam...
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ژورنال
عنوان ژورنال: Applied Microbiology
سال: 1974
ISSN: 0003-6919
DOI: 10.1128/am.28.6.912-914.1974